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The antibody is from purified rabbit serum by affinity purification through sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene has been noticed in lots of tumors, which results in chromosome instability, and so may perhaps contribute to tumorigenesis. This protein was observed to associate with, and be associated with, the phosphorylation of NPAT protein (nuclear protein mapped towards the ATM locus), which participates in mobile-cycle regulated histone gene expression and plays a important position in marketing mobile-cycle development within the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinct isoforms, have been explained. Two extra splice variants were being documented but detailed nucleotide sequence data is not really nevertheless accessible.
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Blocking peptides are peptides that bind particularly to your target antibody and block antibody binding. These peptide typically incorporates the epitope recognized with the antibody.
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This information outlines more feasible optimization of mobile and immunoassay parameters to guarantee the absolute best outcomes are...
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This information outlines even more doable optimization of mobile and immunoassay parameters to guarantee the very best benefits are...
Antibodies sure to the blocking peptide no longer bind to the epitope on the target protein. By evaluating the staining with the blocked antibody as opposed to the antibody by yourself, one can see which staining is certain.
Target Information Cyclin E belongs to the really conserved cyclin relatives, whose customers are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins perform as regulators of CDK kinases. Diverse cyclins exhibit distinct expression and degradation patterns which add to the temporal coordination of each mitotic function. Cyclin E kinds a complex with and capabilities being a regulatory subunit of CDK2, whose activity is necessary for cell cycle G1/S changeover. This protein accumulates with the G1-S phase boundary and is particularly degraded as cells progress through S period.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by each antibodies...
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